The difference is monumental (no pun intended). Find games like Friday Night Funkin, Subject 65, Deadly Night (DEMO), Sleep tight, Octopi Game Online Multiplayer on itch.io, the indie game hosting marketplace. I also had a couple of distracting “indentions” during the development of my hips and ass - they were adjusted & balanced with subsequent filling.įollowing is a variety of “before and after” photos clearly illustrating the outcomes I achieved - comparing some old photos of my buttocks and hips when I still lived as male - to the far more feminine booty I have today. I noticed a couple of small half-inch clumps of nodulation that I could feel by the touch of my fingers within one cheek which I was able to get dissolved with cortisone shots. You will most certainly experience some discomfort and tenderness after each treatment. I feel much more self-confident when naked, in a bathing suit or when wearing a form-fitting dress. My buttocks are soft - yet firm to the touch. I’ve been blessed with no health complications and I’m very pleased with the work. Likewise, its more likely to get clumpy & nodulate - not what we’re after. That’s not healthy and is very unsightly. If you try to fill too much, too quickly - the filler is much more likely to migrate either into your bloodstream or down to your ankles. Not getting too much at once is crucial to achieving lasting, visually appealing & healthy outcomes. Together, these results confirm that biocompatible polymers PEG and PF127 can be used as colloidal stabilizing agents for nanoparticles since they preserve the accessibility of biomolecules to the nanoparticle surface, and they have little effect on the protein corona composition.It took multiple trips to Sao Paulo, Brazil and the sessions had to be spread out over ten years in order to safely achieve my results. On the other hand, BSA had a greater effect on the CSNP protein corona profile compared to other polymers (PEG and PF127). In addition, it was found that the interactions between polymers and CSNPs did not prevent proteins to access the nanoparticles' surface and have minimal effect on the formation of the protein corona when they were incubated in human blood plasma. Although all polymers were adsorbed on the CSNP surface, BSA maintained the CSNP dispersion in the medium by electrosteric stabilization mechanisms, while PEG and PF127 led to the occurrence of depletion forces between the particles. In this work, it was shown that the addition of the polymers (i) Pluronic-F127 (PF127), (ii) polyethylene glycol (PEG) of different molecular weights – 1.5, 12 and 35 kDa – and (iii) the protein bovine serum albumin (BSA) on colloidal silica nanoparticles (CSNPs 135 nm) dispersed in phosphate-buffered saline (PBS) largely alter their colloidal stability through different mechanisms. However, little is known about the influence of these polymers on the interactions between nanoparticles and the biological components of the organism, such as proteins and cells. In these suspensions, polymers can promote high colloidal stability by manifestation of steric and/or depletion forces. NaCl, phosphate buffer), polymers can be added to nanoparticle suspensions that will be used in biomedical applications. To counter the undesired colloidal destabilization of nanoparticles in biologically-compatible media of high ionic strength (i.e.